COURSE
SCIE1046: Fundamentals Of Microbiology With Lab
ASSIGNMENT:
In this unit, you will complete the lab titled Bacterial Isolation. Now it is time to write up your lab report describing the hypothesis and conclusions you drew from the lab experience.
By completing the lab report, you will demonstrate your ability to do the following:
- Describe the lab’s overall objective and make a hypothesis
- Introduce relevant background knowledge on the topic
- Summarize the steps taken in the lab simulation
- Explain any obtained results
- Discuss the conclusions and implications
Download the lab report file:
Lab Report – Bacterial Isolation
The document is a Microsoft Word file. You will type your answers directly into the boxes provided. IMPORTANT: You must submit this completed template file as your Touchstone. Any other submission file or format will be returned ungraded.
In order to foster learning and growth, all work you submit must be newly written specifically for this course. Any plagiarized or recycled work will result in a Plagiarism Detected alert. Review Touchstones: Academic Integrity Guidelines for more about plagiarism and the Plagiarism Detected alert. For guidance on the use of generative AI technology, review Ethical Standards and Appropriate Use of AI.
A. Directions
Step 1: Download and review the lab report file.
Step 2: Complete the simulation and fill in each part of the lab report file as you work through the Activity. Prompts in the lab report will help you develop your responses.
- Follow the process presented to you in the How to Write a Lab Report lessonto ensure you write a thorough lab report entry.
- Review the grading rubric (below) to make sure you have an understanding of how your lab report will be graded.
Step 3: Once you’ve completed the lab simulation and your lab report, review the submission requirements (below) and update your lab report to ensure it meets the recommended requirements.
Solution
1. Describe the overall objective and make a hypothesis.
What is the overall purpose of the experiments or activities? Make a hypothesis. Recall that the hypothesis is a claim that answers a question or makes a prediction about an event that has not yet occurred.
- In essence, the objective of the task is to isolate bacterial colonies from a mixed culture.
Our hypothesis posits that since Salmonella is the suspected pathogen responsible for the infection, it can be cultivated and separated from a mixed sample obtained from the infection source through appropriate methodology.
2. Introduce relevant background knowledge on this topic.
What have you learned in class or researched on your own that would help prepare for this simulation?
- Salmonella, the targeted microorganism for isolation, resides in the gastrointestinal tract of animals and causes Salmonellosis, characterized by its gram-negative nature.
The preferred method for isolation is streaking, where bacteria are inoculated onto a flat, semi-solid growth medium by swiping the inoculating loop back and…
3. Summarize the steps taken in the simulation.
- Samples are collected from a hospitalized patient named Max, who is suffering from a severe bacterial infection.
- Bacteria extracted from Max’s blood were cultured on Salmonella Shigella agar.
- Salmonella was identified on the plate by the presence of distinct black colonies growing on the agar.
- Fecal samples were obtained from the……………………$10